ONC1-0013B inhibits AR activity in vitro. A. ONC1-0013B structure. B. LnCAP cells cultured (10% CSS) for 3 days, then treated with tested compounds in presence of 1nM DHT for 1 day. PSA expression plotted as percentage of vehicle control (DMSO; n=2, mean±SEM). Ki values: ± (ONC1-13B), ± (MDV3100), (ARN-509). Mean±SEM from 5 replicate experiments (except ARN-509). C. LnCAP cells cultured (10% CSS) for 3 days, then treated with tested compounds in presence of 1nM DHT for 5 days. Viable cells plotted as percentage of vehicle control (DMSO; n=2, mean±SEM). IC50 values: 30nM (ONC1-13B), 148nM (MDV3100), 240nM (ARN-509). D. Competitive-binding assay vs AR ligand Fluormone™ (PolarScreen™ Androgen Receptor Competitor Assay). IC50 values: 19nM (DHT), (ONC1-13B), (MDV3100).
Neural injections of Bromodeoxyuridine (BrdU) were applied to males of both groups to test for neurogenesis . Analysis showed that testosterone and dihydrotestosterone regulated adult hippocampal neurogenesis (AHN). Adult hippocampal neurogenesis was regulated through the androgen receptor in the wild-type male rats, but not in the TMF male rats. To further test the role of activated androgen receptors on AHN, flutamide , an antiandrogen drug that competes with testosterone and dihydrotestosterone for androgen receptors , and dihydrotestosterone were administered to normal male rats. Dihydrotestosterone increased the number of BrdU cells, while flutamide inhibited these cells.
Hello Chris Wallace or SARMSstore comments moderator:
I have some champion Siberian Huskies, they are record holders in pushing and pulling competitions for dogs. I would like to provide them with a boost in strength for their next events. I was going to try LG-4033. I have a couple of 220 lbs huskies and three 132 lbs female huskies. I was going to give the males 10mg and the females 5mg. Does this sound about right. Also, I keep reading about cycling. As I understand cycling as it applies to oral testosterones that is because of the alkyd molecule at the 17a position which caused liver damage but allowed the testosterone to make multiple passes thru the liver.
Since LG-4033 is a SARMS, there is no Alkyd molecule to cause liver dysfunction, correct? Is it really necessary to cycle the SARMS then? I really care about my Huskies and only want the best for them.
These are serious questions and I hope you take the time to respond.
Thanks, Husky Trainer.